E3ISPM21000KPA (Discontinued) Microscopy Cameras/Microscope Cameras
Product Introduction
21MP large format color CMOS camera featuring 4/3" large-size IMX269 sensor with 3.3 µm pixels providing excellent light-gathering capability. High sensitivity of 399 mV delivers outstanding performance in low-light conditions, supporting frame rates from 17 fps at full resolution to 192 fps in high-speed mode. 12-bit hardware ISP processing ensures image quality while being particularly suitable for fluorescence microscopy, darkfield imaging and other applications requiring high sensitivity.
Key Features
- 21MP high resolution (5280×3954)
- 4/3" large format sensor with wide field of view
- IMX269 CMOS with 3.3 µm large pixel design
- High sensitivity 399 mV with excellent low-light performance
- 12-bit Ultra-Fine hardware ISP engine
- Support for square and standard format switching
- High-speed mode reaching 192 fps@584×438
- Hardware real-time color processing
- Parallel A/D conversion with low-noise design
- Flexible 8/12-bit output
- USB3.0 high-speed data transmission
- Suitable for fluorescence and low-light microscopy imaging
- Zinc-aluminum alloy CNC machined housing
- Comprehensive SDK development support
Product Details
| Key Parameters | |
| Model | E3ISPM21000KPA (Discontinued) |
| Sensor | IMX269 (C) |
| Effective Pixels / Resolution | 21M (5280×3954) |
| Frame Rate (Full Resolution) | 17 fps@5280×3954; 17 fps@3952×3952; 56 fps@2640×1976; 67 fps@1760×1316; 192 fps@584×438 |
| Shutter Type | Rolling Shutter |
| Color Type | Color |
| Imaging Performance | |
| Pixel Size | 3.3 µm × 3.3 µm |
| Sensor Size | 17.4 mm × 13.0 mm |
| Diagonal | 4/3" (21.72 mm) |
| Dynamic Range | TBD |
| Bit Depth | 8/12-bit |
| Sensitivity | 399 mV with 1/30s; 0.1 mV with 1/30s |
| Interface & Mechanical | |
| Data Interface | USB3.0 |
| GPIO | TBD |
| Lens Mount | C-mount |
| Dimensions | TBD |
| Weight | TBD |
| Power Supply | USB3.0 interface power (compatible with USB2.0) |
| Environment & Certification | |
| Operating Temperature / Humidity | -10 °C ~ +50 °C / 30%~80%RH |
| Storage Temperature / Humidity | -20 °C ~ +60 °C / 10%~60%RH |
| Operating System | Windows/Linux/macOS/Android multi-platform SDK (native C/C++, C#/VB.NET, Python, Java, DirectShow, TWAIN, etc.) |
| Certification | CE, FCC |
Product Overview
E3ISPM21000KPA (Discontinued) is a digital camera specifically designed for microscopic imaging applications, featuring a IMX269 (C) high-performance image sensor with the following characteristics:
- Exceptional Imaging Quality: 21M (5280×3954) resolution with 3.3 µm × 3.3 µm pixel size, sensor size of 17.4 mm × 13.0 mm , ensuring clear detail presentation
- Imaging Characteristics: Features Rolling Shutter design, supports color imaging, suitable for brightfield, darkfield, phase contrast, fluorescence and other microscopy observation methods
- High-Speed Data Transfer: Utilizes USB3.0 interface with maximum frame rate up to 17 fps@5280×3954; 17 fps@3952×3952; 56 fps@2640×1976; 67 fps@1760×1316; 192 fps@584×438 , supporting real-time preview and rapid capture
- Color Reproduction: 8/12-bit color depth with dynamic range reaching TBD , accurately reproducing sample colors
- Sensitivity Performance: Excellent low-light performance with sensitivity of 399 mV with 1/30s; 0.1 mV with 1/30s , suitable for low-light sample observation
- Optical Compatibility: Standard C-mount interface, compatible with various microscope brands, optional 0.5×/0.63×/1× adapters available
- Compact Design: Overall dimensions of only TBD , weighing TBD , powered by USB3.0 interface power (compatible with USB2.0) , no additional power supply required
- Software Support: Includes professional microscopy imaging software ToupView, supports Windows/Linux/macOS/Android multi-platform SDK (native C/C++, C#/VB.NET, Python, Java, DirectShow, TWAIN, etc.) systems, provides measurement, annotation, stitching, focus stacking and other functions, supports C/C++, C#, Python, ImageJ, μManager development environments
Core Performance Indicators
Resolution
21M (5280×3954)
Frame Rate
Up to 17 fps@5280×3954; 17 fps@3952×3952; 56 fps@2640×1976; 67 fps@1760×1316; 192 fps@584×438
Pixel Size
3.3 µm × 3.3 µm
Data Interface
USB3.0
Microscopic Imaging Features
Professional Microscopy Applications
Optimized for biomedical, materials science, and industrial inspection microscopy needs, supports brightfield, darkfield, phase contrast, fluorescence and other observation methods
Excellent Color Reproduction
Precise color reproduction algorithms and white balance control ensure accurate and true sample colors, meeting professional needs such as pathological diagnosis
Measurement and Analysis Functions
Bundled software provides measurement tools for length, area, angle, etc., supports annotation and statistical analysis, meeting quantitative analysis requirements
Image Processing Capabilities
Supports real-time image enhancement, focus stacking, image stitching and other advanced functions, improving observation effects and imaging quality
Why Choose This Camera
The E3ISPM21000KPA (Discontinued) microscopy camera, with its excellent imaging quality, convenient operation experience, and professional software support, is an ideal choice for microscope digital upgrades. Whether for biomedical research, material analysis, or industrial inspection, it provides clear, accurate, and reliable microscopic image recording and analysis solutions.
E3ISPM21000KPA (Discontinued) Product Manual
PDF format, includes detailed technical specifications and dimensional structure
SDK Development Kit
Supports Windows, Linux, macOS and other platforms
3D Model Files
STEP format, for mechanical design integration
Package Contents #
Standard configuration and packing details for E3ISPM series hardware ISP USB 3.0 CMOS cameras
Standard Packing List
- Outer carton: L 52 cm × W 32 cm × H 33 cm (20 pcs, approximately 12-17 kg per carton)
- Inner box: L 15 cm × W 15 cm × H 10 cm (0.58-0.60 kg per box)
- One E3ISPM series camera
- High-speed USB 3.0 Type-A to Type-B gold-plated cable (2.0 m)
- CD (drivers and application software, Ø 12 cm)
Optional Accessories
- Adjustable eyepiece tube adapters
Dia. 23.2 mm eyepiece tube to C-mount: 108001/AMA037, 108002/AMA050, 108003/AMA075, 108004/AMA100
Dia. 31.75 mm eyepiece tube to C-mount: 108008/ATA037, 108009/ATA050, 108010/ATA075, 108011/ATA100 - Fixed eyepiece tube adapters
Dia. 23.2 mm eyepiece tube to C-mount: 108005/FMA037, 108006/FMA050, 108007/FMA075, 108008/FMA100
Dia. 31.75 mm eyepiece tube to C-mount: 108011/FTA037, 108012/FTA050, 108013/FTA075, 108014/FTA100 - 108015 (Dia. 23.2 mm to 30.0 mm ring) - adapter ring for 30 mm eyepiece tubes
- 108016 (Dia. 23.2 mm to 30.5 mm ring) - adapter ring for 30.5 mm eyepiece tubes
- 108017 (Dia. 23.2 mm to 31.75 mm ring) - adapter ring for 31.75 mm eyepiece tubes
- Stage micrometers
106011/TS-M1 (X = 0.01 mm/100 Div.)
106012/TS-M2 (X, Y = 0.01 mm/100 Div.)
106013/TS-M7 (X = 0.01 mm/100 Div., 0.10 mm/100 Div.)
Product Dimensions #
Outline reference for E3ISPM series hardware ISP CMOS cameras
Frequently Asked Questions
Learn more about the professional features of E3ISPM series microscopy imaging cameras
- Real-Time Processing: Ultra-Fine™ HISP VP completes demosaicing, auto exposure, white balance, and color correction inside the camera
- High-Quality Output: Direct output of 8/12-bit high-quality images without post-processing
- Low CPU Usage: Dramatically reduces computer CPU load, improving overall system efficiency
- Zero-Latency Imaging: Reduces backend processing delays for true "instant capture"
Hardware ISP Integration: Unique in its class for integrating 12-bit ISP within the camera body for more stable performance.
Research-Grade Image Quality: Maintains professional imaging quality while effectively controlling costs, outstanding cost-performance ratio.
Complete Ecosystem: Provides ToupView/ToupLite software and open-source SDK, reducing secondary development costs.
In-Depth Product Introduction
Ultra-Fine™ HISP VP Hardware ISP Engine
Features our proprietary 12-bit hardware image signal processor that performs real-time demosaicing, auto exposure control, auto white balance, and color correction inside the camera. Compared to software ISP, processing speed is improved by more than 10× with latency reduced to microsecond levels, achieving true "what you see is what you get" at the microscope.
Sony Exmor Back-Illuminated CMOS Technology
Features the latest Sony Exmor back-illuminated CMOS sensors with quantum efficiency >70% and dark current <0.1 e⁻/pix/s. The back-illuminated structure significantly improves photoelectric conversion efficiency, particularly suitable for low-light and fluorescence imaging applications, delivering clear images even under extremely low illumination conditions.
USB 3.0 High-Speed Data Transfer
Utilizes USB 3.0 SuperSpeed interface with bandwidth up to 5 Gbps, maintaining real-time preview at over 10 fps even for 45-megapixel models. No additional power supply required, plug-and-play compatibility with various microscope systems.
Flexible Imaging Modes
Supports arbitrary ROI area selection, 2×2/3×3/4×4 pixel binning, and 8-bit/12-bit data depth switching. Exposure time adjustable from 0.1 ms to 15 s, meeting comprehensive requirements from high-speed dynamic observation to long-term accumulative exposure.
Complete Hardware and Software Ecosystem
Provides multi-platform support for Windows/Linux/macOS, bundled with ToupView professional acquisition software and ToupLite lightweight tools. Open SDK interfaces including C/C++, C#/VB.NET, DirectShow, and Twain facilitate system integration and secondary development. All-metal CNC housing design with no fans or mechanical moving parts ensures long-term stable operation.
Key Application Areas
E3ISPM series applications across various microscopy imaging fields
E3ISPM Series Core Advantages
12-Bit Hardware ISP
Ultra-Fine™ HISP VP real-time processing engine
Sony Exmor CMOS
Back-illuminated sensors, quantum efficiency >70%
USB 3.0 High-Speed Transfer
45 MP maintains 10+ fps preview
Flexible Imaging Modes
Arbitrary ROI + multiple pixel binning
Cross-Platform SDK
Windows/Linux/macOS full coverage
Industrial-Grade Design
All-metal CNC housing, fanless silent
Ultra-Low Dark Current
<0.1 e⁻/pix/s, suitable for long exposure
High Cost-Performance
Research-grade image quality, controlled costs